Western blot analysis of EIF2S1 in different various lysates. Lane 1: HepG2 cell lysate; Lane 2: A431 cell lysate; Lane 3: Raji cell lysate; Lane 4: Hela cell lysate; Lane 5: PC-12 cell lysate; Lane 6: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa. Observed band size: 36 kDa. Exposure time: 21 seconds; 12% SDS-PAGE gel. Primary antibody EIF2S1 polyclonal antibody (Product # PA5-144655) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.